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| Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R.. |
| The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and... |
| Tipo: Text |
Palavras-chave: Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00101/21195/19415.pdf |
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| CASELI,LUCIANO. |
| ABSTRACT In this review we discuss about the immobilization of enzymes in Langmuir-Blodgett films in order to determine the catalytic properties of these biomacromolecules when adsorbed on solid supports. Usually, the conformation of enzymes depends on the environmental conditions imposed to them, including the chemical composition of the matrix, and the morphology and thickness of the film. In this review, we show an outline of manuscripts that report the immobilization of enzymes as LB films since the 1980’s, and also some examples of how the surface properties of the floating monolayer prepared previously to the transfer to the solid support are important to determine the efficiency of the resulting device. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Langmuir-Blodgett; Enzymes; Biosensors; Air-water interface. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200631 |
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| Villalobos,Patricio; Chávez,María Isabel; Olguín,Yusser; Sánchez,Elizabeth; Valdés,Erika; Galindo,Raúl; Young,Manuel E. |
| The inadequate treatments given to the served waste water which are disposal to the rivers and sea coast are the major sources of faecal Microorganisms and enteric bacterial pathogens. They are among the most serious effects of water pollution bringing risks on public health. None of the current methods for detection of pathogens offer real-time on site solutions, are capable of delivering a simple visual detection signal, or can be easily instrumented as an indicator of the presence of a pathogen in water. The use of lipid vesicles incorporating Polydiacetylenes (PDAs) for the development of biosensors for “real-time” detection of pathogens has become an alternative, due to its potential for simple colorimetric response against harmful... |
| Tipo: Journal article |
Palavras-chave: Bacterial detection; Biosensors; Lipid vesicles; PDA. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100004 |
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| Serna Cock,Liliana; Zetty Arenas,Ana María; Ayala Aponte,Alfredo. |
| Biosensors are an important alternative in the food industry to ensure the quality and safety of products and process controls with effective, fast and economical methods. Their technology is based on a specific biological recognition element in combination with a transducer for signal processing. The use of enzymatic biosensor technology in food processing, quality control and on-line processes is promising compared to conventional analytical techniques, as it offers great advantages due to size, cost, specificity, fast response, precision and sensitivity. This article reviews the development and use of some enzyme biosensors in the food industry, describes the most important application areas and analyzes the current situation and future possibilities.... |
| Tipo: Journal article |
Palavras-chave: Biosensors; Enzymes; Analysis in food; Safety; Quality; Control processes. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200017 |
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